I'm trying to use uWSGI with a virtual env in order to use the Trac bug tracker on it. I've installed system-wide uwsgi via pip. Next, I've installed trac in a virtualenv $ virtualenv venv $ . venv/bin/activate $ pip install trac I've then written a simple uWSGI configuration script: [uwsgi] master = true processes = 1 socket = localhost:3032 home = /srv/http/trac/venv/ no-site = true gid = www-data uid = www-data env = TRAC_ENV=/srv/http/trac/projects/my_project module = trac.web.main:dispatch_request But when I try to launch it, it fails: $ uwsgi --http :8000 --ini /etc/uwsgi/vassals-available/my_project.ini --gid www-data --uid www-data ... Set PythonHome to /srv/http/trac/venv/ ... *** Operational MODE: single process *** ImportError: No module named trac.web.main unable to load app 0 (mountpoint='') (callable not found or import error) I think uWSGI isn't using the virtual env. When inside the virtual env, I can import trac.web.main without having an ImportError. How can I do that ? Thanks