I want to get multiple file by parsing a input file Through Java.
The Input file contains many fasta format of thousands of protein sequence and I want to generate raw format(i.e., without any comma semicolon and without any extra symbol like "", "[", "]" etc) of each protein sequence.
A fasta sequence starts form "" symbol followed by description of protein and then sequence of protein.
For example ? lcl|NC_000001.10_cdsid_XP_003403591.1 [gene=LOC100652771]
[protein=hypothetical protein LOC100652771] [protein_id=XP_003403591.1] [location=join(12190..12227,12595..12721,13403..13639)]
MSESINFSHNLGQLLSPPRCVVMPGMPFPSIRSPELQKTTADLDHTLVSVPSVAESLHHPEITFLTAFCL
PSFTRSRPLPDRQLHHCLALCPSFALPAGDGVCHGPGLQGSCYKGETQESVESRVLPGPRHRH
Like above formate the input file contains 1000s of protein sequence. I have to generate thousands of raw file containing only individual protein sequence without any special symbol or gaps.
I have developed the code for it in Java but out put is : Cannot open a file followed by cannot find file.
Please help me to solve my problem.
Regards
Vijay Kumar Garg
Varanasi
Bharat (India)
The code is
/*Java code to convert FASTA format to a raw format*/
import java.io.*;
import java.util.*;
import java.util.regex.*;
import java.io.FileInputStream;
// java package for using regular expression
public class Arrayren
{
public static void main(String args[]) throws IOException
{
String a[]=new String[1000];
String b[][] =new String[1000][1000];
/*open the id file*/
try
{
File f = new File ("input.txt");
//opening the text document containing genbank ids
FileInputStream fis = new FileInputStream("input.txt");
//Reading the file contents through inputstream
BufferedInputStream bis = new BufferedInputStream(fis);
// Writing the contents to a buffered stream
DataInputStream dis = new DataInputStream(bis);
//Method for reading Java Standard data types
String inputline;
String line;
String separator = System.getProperty("line.separator");
// reads a line till next line operator is found
int i=0;
while ((inputline=dis.readLine()) != null)
{
i++;
a[i]=inputline;
a[i]=a[i].replaceAll(separator,"");
//replaces unwanted patterns like /n with space
a[i]=a[i].trim();
// trims out if any space is available
a[i]=a[i]+".txt";
//takes the file name into an array
try
// to handle run time error
/*take the sequence in to an array*/
{
BufferedReader in = new BufferedReader (new FileReader(a[i]));
String inline = null;
int j=0;
while((inline=in.readLine()) != null)
{
j++;
b[i][j]=inline;
Pattern q=Pattern.compile(">");
//Compiling the regular expression
Matcher n=q.matcher(inline);
//creates the matcher for the above pattern
if(n.find())
{
/*appending the comment line*/
b[i][j]=b[i][j].replaceAll(">gi","");
//identify the pattern and replace it with a space
b[i][j]=b[i][j].replaceAll("[a-zA-Z]","");
b[i][j]=b[i][j].replaceAll("|","");
b[i][j]=b[i][j].replaceAll("\\d{1,15}","");
b[i][j]=b[i][j].replaceAll(".","");
b[i][j]=b[i][j].replaceAll("_","");
b[i][j]=b[i][j].replaceAll("\\(","");
b[i][j]=b[i][j].replaceAll("\\)","");
}
/*printing the sequence in to a text file*/
b[i][j]=b[i][j].replaceAll(separator,"");
b[i][j]=b[i][j].trim();
// trims out if any space is available
File create = new File(inputline+"R.txt");
try
{
if(!create.exists())
{
create.createNewFile();
// creates a new file
}
else
{
System.out.println("file already exists");
}
}
catch(IOException e)
// to catch the exception and print the error if cannot open a file
{
System.err.println("cannot create a file");
}
BufferedWriter outt = new BufferedWriter(new FileWriter(inputline+"R.txt", true));
outt.write(b[i][j]);
// printing the contents to a text file
outt.close();
// closing the text file
System.out.println(b[i][j]);
}
}
catch(Exception e)
{
System.out.println("cannot open a file");
}
}
}
catch(Exception ex)
// catch the exception and prints the error if cannot find file
{
System.out.println("cannot find file ");
}
}
}
If you provide me correct it will be much easier to understand.
